In vitro assessment of stearyl triphenyl phosphonium toxicity in drug-resistant tumor cells

Introduction : The triphenyl phosphonium residue is a well-documented mitochondriotropic that has been shown to improve the accumulation of biomolecules in mitochondria. Stearyl (STPP) modified liposomes have facilitate selective various mitochondria resulting improved effect in-vitro and in-vivo. More recently, STPP was reported higher toxicity towards drug resistant ovarian cancer cell line compare non-drug line. purpose this study further investigate using multiple lines. Methods incorporated into phosphatidylcholine cholesterol thin film hydration method. Mean particle size zeta potential measured dynamic light scattering. 5,5,6,6′-tetrachloro-1,1′,3,3′ tetraethylbenzimi-dazoylcarbocyanine iodide (JC-1) dye assay used as an indicator mitochondrial membrane tested Cytotoxicity preparations different lines determined microscopy CellTiter 96 ® AQueous One Solution Cell Proliferation assay. Results JC-1 confirmed drug-resistant had significantly than Higher cytotoxicity seen when but not dissolved dimethyl sulfoxide (DMSO). showed comparable profile known oxidative phosphorylation uncoupler carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone (FCCP). Discussion Taken together, data suggest influenced by presence liposomal lipids acts way similar therefore more toxic cells rely on maintain their viability.